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A Rapid Method of Estimation of Lactic Acid in Skim Milk Powder

Authors: A.J. Lawrence


A method was developed for the rapid determination of the content of lactic acid in skim milk powder. Interfering substances were removed from reconstituted milk by treatment with calcium hydroxide and copper sulphate, followed by centrifugation. Concentrated sulphuric acid was used to oxidise the lactic acid in an aliquot of the supernatant to acetaldehyde, which was estimated by means of its colour reaction with p-hydroxy diphenyl. The method was suitable for the determination of lactic acid contents in the range 20-120 mg/100g powder, and should extend to a lower limit of 10 mg /100 g.

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