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A Method for the Determination of Total Iodine in Milk

Authors: N. Ahmad


Milk was wet-ashed with potassium dichromate and sulphuric acid to oxidize iodine to iodate which was then treated with phosphorous acid. The liberated iodine was distilled and trapped in ammonium hydroxide. Iodine in the distillate was determined by the selective oxidation of leuco-crystal violet to crystal violet by hypoiodous acid, formed by the action of N-chlorosuccinimide/succinimide on ammonium iodide. The method is simple, reliable and the colour developed is stable. The recovery of added iodine was 88% with a mean standard deviation of 7.5.

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