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The use of 2, 2'-diphenyl-1-picrylhydrazyl for measuring antioxidant properties of butterfat

Authors: P.W. Parodi

Abstract:

The possible use of the stable free radical 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) for measuring antioxidant properties of butterfat was investigated. There was a lack of correlation between the extent of reduction of DPPH and either tocopherol content or stability of the fat. As DPPH also reacts with hydroperoxides it is not considered a suitable reagent for measuring the antioxidant properties of butterfat. As it reacts with most commercial antioxidants it may be used to detect their presence in a fat provided the history of the fat is known.

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