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A collaborative trial for the establishment of a skim milk powder reference protein standard

Authors: P.G. Wiles and I.K. Gray


A collaborative trial involving 13 dairy laboratories was conducted to determine the total organic nitrogen (TN) content of samples of skim milk powder with a view to using such a material as a protein reference. A dry basis reference value of 6.61% TN with confidence limits of 0.10% TN (reproducibility) and 0.054% TN (repeatability) was obtained for the Kjeldahl-based instruments. Comparison of this value with a value determined from limited data using the Dumas technique was made. A higher mean value for the Dumas method was found. The methodology can be used to develop reference materials for a variety of protein-rich dairy products.

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